ccdc57 orchestrates synchronized beating of motile cilia in the central canal.(a) confocal images showing the localization of basal bodies in the floor plate cells at 2 dpf and 5 dpf. basal bodies were stained with anti-γ tubulin (gama-tub, green) antibody, and nuclei were counterstained with dapi in blue. the white dotted line connects the center of cells. (b) statistical analysis showing the angles of basal bodies with the anterior–posterior (a–p) axis as illustrated in the diagram. (c) still images showing kymographs of floor plate cilia movement in 5 dpf of wild type and ccdc57 mutant. (d) beating frequency of floor plate cilia in wild type and ccdc57 mutants. (e) statistical analysis showing the rotation angles of cilia in floor plate. the anterior and posterior angles were measured as illustrated in the diagram. (f, g) relative tilting directions of floor plate cilia in wild type and ccdc57 mutants. the tilting direction was evaluated by the bisector of each angles. (h) confocal images showing relative localization of dvl protein (green) and basal body (gama-tub, red) in floor plate cells. (i, j) subcellular localization of dvl protein (green) in the floor plate ependymal cells. the statistical results were shown in panel j. (k) confocal images showing the localization of gfp-prickle (green) in floor plate ependymal cells as indicated. (l) confocal images showing the distribution of dvl protein (green) on ependymal cells of adult zebrafish. the displacement distance of dvl vesicles was also shown on the right. scale bars: 5 μm in panel a; 5 μm in panels h and i; 10 μm in panel k; 7.5 μm in panel l. the data underlying the graphs shown in the figure can be found in s1 data.,ccdc57 orchestrates synchronized beating of motile cilia in the central canal.(a) confocal images showing the localization of basal bodies in the floor plate cells at 2 dpf and 5 dpf. basal bodies were stained with anti-γ tubulin (gama-tub, green) antibody, and nuclei were counterstained with dapi in blue. the white dotted line connects the center of cells. (b) statistical analysis showing the angles of basal bodies with the anterior–posterior (a–p) axis as illustrated in the diagram. (c) still images showing kymographs of floor plate cilia movement in 5 dpf of wild type and ccdc57 mutant. (d) beating frequency of floor plate cilia in wild type and ccdc57 mutants. (e) statistical analysis showing the rotation angles of cilia in floor plate. the anterior and posterior angles were measured as illustrated in the diagram. (f, g) relative tilting directions of floor plate cilia in wild type and ccdc57 mutants. the tilting direction was evaluated by the bisector of each angles. (h) confocal images showing relative localization of dvl protein (green) and basal body (gama-tub, red) in floor plate cells. (i, j) subcellular localization of dvl protein (green) in the floor plate ependymal cells. the statistical results were shown in panel j. (k) confocal images showing the localization of gfp-prickle (green) in floor plate ependymal cells as indicated. (l) confocal images showing the distribution of dvl protein (green) on ependymal cells of adult zebrafish. the displacement distance of dvl vesicles was also shown on the right. scale bars: 5 μm in panel a; 5 μm in panels h and i; 10 μm in panel k; 7.5 μm in panel l. the data underlying the graphs shown in the figure can be found in s1 data.